The connecting theme throughout Dr. Prlic's research career has been my interest in lymphocyte differentiation. This started initially at the University of Salzburg, Austria, followed by his Ph.D. training at the University of Minnesota in the laboratory of Dr. Stephen Jameson. As a postdoctoral fellow at the University of Washington, he had the opportunity to establish his own T cell and NK cell research projects in the laboratory of Dr. Michael Bevan with a focus on T cell and NK cell responses in the context of infectious diseases. When Dr. Prlic started my own laboratory at the Fred Hutchinson Cancer Research Center in 2011, he continued his T cell work in the context of vaccination and infectious diseases. Through a close collaboration with Dr. Raphael Gottardo (FHCRC) his lab has started to examine human T cell subsets from blood and mucosal tissues using different single-cell gene expression analysis strategies. Our overall goal is to understand how T cell fate and function are controlled in healthy and inflamed tissues and following infection with HIV to identify how these responses can be manipulated for therapeutic purposes.