Anne Gaelyn Kasmar MD MSc anne.kasmar@gatesfoundation.org Education 1995 B.A. with honors, Comparative Literature (French and German). Brown University, Providence Rhode Island 2000 MD degree. University of California San Francisco School of Medicine. San Francisco, California 2004 MSc. Immunology. London School of Hygiene & Tropical Medicine London, United Kingdom Fall 2014 Certificate in Management and Leadership, MIT Sloan School of Management Innovation, entrepreneurship & project management Cambridge, MA Summer 2016 Advanced Negotiation, Program on Negotiation and Harvard Law School Cambridge, MA Postdoctoral Training 06/2000-07/2003 Intern and Resident, Department of Medicine, Massachusetts General Hospital. (MGH) Boston, MA 08/2003-06/2004 Warren-Whitman Richardson Fellow, London School of Hygiene 07/2004-07/2005 Clinical Fellow, MGH-Brigham Combined Program in Infectious Disease 07/2005-06/2006 Chief Resident, Department of Medicine, MGH 06/2006-07/2008 Research Fellow, MGH-Brigham Combined Program in Infectious Disease 07/2008-12/2014 Instructor in Medicine, Harvard Medical School Employment 02/2015- Program Officer, TB vaccines, The Bill and Melinda Gates Foundation Major Administrative Leadership Appointments 2008 New England Tuberculosis retreat, co-organizer 2009 New England Tuberculosis retreat, lead organizer 2010 New England Tuberculosis retreat, co-organizer 2011 New England Tuberculosis retreat, co-organizer 2012-2014 Chair, Gordon Research Seminar, Immunochemistry & Immunobiology Committee Service 09/2011-08/2013 Member, Partners Institutional Review Board. Boston MA 10/2015-9/2018 Member, Research Committee, Infectious Diseases Society of America Professional Societies 07/2008-present Member, the Infectious Diseases Society of America (IDSA) 08/2013-present Member, the American Association of Immunologists Grant Review Activities 12/2012 Ad hoc reviewer, Harvard Center for AIDS Research Scholar/Feasibility awards Editorial Activities 01/2008-12/2009 Image Associate, New England Journal of Medicine Ad hoc reviewer The Journal of Infectious Diseases Clinical Infectious Diseases The Journal of Immunology Honors and Prizes 1995 Gordon Rohde Dewart Award, Brown University 1997 French Foundation for Medical Research and Education Spitalny Award, UCSF 1999 Luce Scholar in Hat Yai, Thailand. The Henry Luce Foundation 2004 Warren Whitman Richardson Fellowship, Harvard Medical School 2006 Edward H. Kass Award for Clinical Excellence, Massachusetts ID Society 2007 ID fellows program participant at the 47th Interscience Conference on Antimicrobial Agents and Chemotherapy. Chicago, Illinois 2008 Keystone Symposium: NK/NKT cell biology travel award recipient 2011 Harvard Catalyst: Leadership strategies for the researcher, invited attendee Fall 2014 TEDMED, Front Line Scholar Summer 2016 Advanced Course on Vaccinology, Fondation Merieux & Universite de Geneve Funding information 2009-2010 CD1-restricted T cells in TB Harvard Global Health Institute career development award Research Fellow ($75,000) The goal of the study was to use newly generated CD1 tetramers to demonstrate glycolipid-specific T cells in the peripheral blood of Boston TB patients. 2009-2011 Detection of M. tb Lipid-Specific T Cells in South African Adults Harvard Global Health Institute travel award Principal Investigator ($25,000) The goal of the award was to extend CD1 tetramer studies and the discovery of glycolipid-specific T cells from a small cohort of HIV negative subjects in Boston to a larger cohort of TB and HIV co-infected South African adults. 2010-2015 Mycolate-specific T cell function in human immunity to M. tuberculosis NIAID K08AI089858, mentored-training grant Principal Investigator ($638,200) The goal of this award is to use CD1 tetramers generated by the candidate to investigate the immunological functions of glycolipid-specific T cells during human infection with Mycobacterium tuberculosis (in Boston) and during co- infection with TB and HIV (at the KwaZulu Natal Research Institute for TB and HIV (in Durban, South Africa). 2012-2014 Immunologic Functions of Mycolate-specific T cells in TB/HIV The KwaZulu Natal Research Institute for TB and HIV Principal Investigator ($50,000) The purpose of this grant is to determine the impact of HIV co-infection on the frequency, phenotype and function of human glycolipid-specific T cells. 2013-2016 Immunization of Glycolipid-specific T cells in vivo in Guinea Pigs The Bill and Melinda Gates Foundation Co-investigator ($2,733,555) The purpose of this grant is to determine whether vaccination with synthetic mycobacterial glycolipids leads to durable immunologic memory in guinea pigs. The candidate will generate guinea pig CD1 tetramers in order to make specific, single-cell measurements of glycolipid-specific T cells responding during vaccination. Clinical Supervisory and Training Responsibilities 2009-2014 Attending physician, MGH Infectious Diseases consult service, 4 weeks per year Practice Activities 04/2009-12/2014 One four week block per year attending in Infectious Diseases at MGH 07/2009-12/2014 One half-day per week in the outpatient MGH Mycobacterial Center Report of Technological and Other Scientific Innovations Generated the first human CD1a, CD1b and CD1c tetramers which allow single-cell detection of human T cells that recognize mycobacterial lipids as cognate antigens in subjects with latent or active TB. Report of Scholarship Research investigations 1. Vyas JM, Kasmar A, Chang HR, Holden J, Hohmann E. Abdominal abscesses due to actinomycosis after laparoscopic cholecystectomy: case reports and review. Clin Infect Dis. 2007 Jan 15; 44(2):e1-4. PMID: 17173208 4 citations ISI 02/19/14 2. Young DC, Kasmar A, Moraski G, Cheng TY, Waltz AJ, Hu J, Xu X, Endres GW, Uzieblo A, Zajonc D, Costello CE, Miller MJ, Moody DB. Synthesis of dideoxymycobactin antigens presented by CD1A reveals T cell fine specificity for natural lipopeptide structures. J Biol Chem 2009; 284 (37):25087-96). PMID: 19605355 PMCID: PMC2757212 3 citations ISI 02/19/14 3. Yakimchuk K, Roura-Mir C, Magalhaes KG, de Jong A, Kasmar AG, Granter SR, Budd R, Steere A, Pena-Cruz V, Kirschning C, Cheng TY, Moody DB. Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-ß. Eur J Immunol. 2011 Mar; 41(3):694-705. doi: 10.1002/eji.201040808. Epub 2011 Jan 18. PMID: 21246541; PMCID: PMC3082368. 4 citations ISI 02/19/14 4. Mureithi MW, Cohen K, Moodley R, Poole D, Mncube Z, Kasmar A, Moody DB, Goulder PJ, Walker BD, Altfeld M, Ndung'u T. Impairment of CD1d- restricted natural killer T cells in chronic HIV type 1 clade C infection. AIDS Res Hum Retroviruses. 2011 May; 27(5):501-9. PMID: 20942750; PMCID: PMC3083726. 4 citations ISI 02/19/14 5. Kasprowicz VO, Mitchell JE, Chetty S, Govender P, Huang KH, Fletcher HA, Webster DP, Brown S, Kasmar A, Millington K, Day C L, Mkhwanazi N, McClurg C, Chonco F, Lalvani A, Walker BD, Ndung'u T, Klenerman P. A molecular assay for sensitive detection of pathogen-specific T-cells. PLOS One 2011; 6(8):e20606. Epub 2011 Aug 11. PMID: 21853018 12 citations ISI 02/19/14 6. Kasmar AG, van Rhijn I, Cheng TY, Turner MT, Seshadri C, Schiefner A, Kalathur RC, Annand JW, de Jong A, Shires J, Leon L, Brenner MB, Wilson IA, Altman JD, Moody DB. CD1b tetramers bind ...T cell receptors to identify a mycobacterial glycolipid-reactive T cell repertoire in humans. J Exp Med 2011 Aug 29;208(9):1741-7. PMID: 21807869 PMCID:PMC3171094 17 citations ISI 02/19/14 7. León L, Tatituri RV, Grenha R, Sun Y, Barral DC, Minnaard AJ, Bhowruth V, Veerapen N, Besra GS, Kasmar A, Peng W, Moody DB, Grabowski GA, Brenner MB. Saposins utilize two strategies for lipid transfer and CD1 antigen presentation. Proc Natl Acad Sci U S A. 2012 Mar 20; 109(12):4357-64. PubMed PMID: 22331868. PMCID: PMC3311357 6 citations ISI 02/19/14 8. Mitchell JE, Chetty S, Govender P, Pillay M, Jaggernath M, Kasmar A, Ndung'u T, Klenerman P, Walker BD, Kasprowicz VO. Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals. PLoS One. 2012; 7(6):e37920. Epub 2012 Jun 7. PMID: 22685549 PMCID: PMC3369919 1 citations ISI 02/19/14 9. Ly D, Kasmar AG, Cheng TY, de Jong A, Huang S, Bhatt A, van Summeren RP, Minnaard AJ, Jacobs WR, Porcelli SA, Moody DB. Cellular antigen processing generates a sugar free antigen that binds to CD1c tetramers. J Exp Med 2013; 210(4):729-41. PMID: 23530121 PMCID: PMC3620358 4 citations ISI 02/19/14 10. van Rhijn I, Kasmar AG, de Jong A, Gras S, Bhati M, Godfrey DI, Altman JD, Rossjohn J, Moody DB. An invariant human T cell population targets mycobacterial antigens presented by CD1b. Nat Immunol; 2013 (14)7:706-13. PMID: 23727893 PMCID: PMC3723453 8 citations ISI 02/19/14 11. Kasmar AG, van Rhijn, I, Magalhaes KG, Young DC, Cheng TY, Turner MT, Schiefner A, Kalathur RC, Wilson IA, Bhati M, Gras S, Rossjohn J, Shires J, Jakobsen S, Altman JD, Moody DB. CD1a tetramers and dextramers identify human lipopeptide-specific T cells ex vivo. J Immunol 2013; 191:4499-4503. PMID: 24089190 PMCID: PMC3845436 0 citations ISI 02/19/14 12. de Jong A, Cheng TY, Huang S, Gras S, Birkinshaw RW, Kasmar AG, van Rhijn I, Peña-Cruz V, Ruan D, Altman JD, Rossjohn J, Moody DB. CD1a autoreactive T cells recognize natural skin oils that function as headless antigens. Nat Immunol; 2014 Feb; 15(2):177-85. PMID: 24362891 0 citations ISI 02/19/14 13. van Rhijn I, Gherardin N, Kasmar AG, Pellicci D, Gras S, Jager W, Tan LL, Bhati M, Godfrey D, Rossjohn J, Moody DB. T cell receptor bias and affinity define two compartments of the CD1b-glycolipid specific T cell repertoire. J Immunol 2014; 192:4054-4060. PMID: 24683194 14. Kasprowicz, VO, Cheng TY, Ndung’u T, Sunpath H, Moody DB, Kasmar AG. HIV disrupts human T cells that target mycobacterial glycolipids. J Infect Dis, in press. Non-peer reviewed scientific or medical publications/materials in print or other media 1. Steele DJR, Kasmar AG, Guimares AS and JP Dekker. Case records of the Massachusetts General Hospital. A 72-Year-Old Woman with Nausea and Abdominal Distension after Peritoneal Dialysis. NEJM 2013; 368(4): 375-85. PMID: 23343067 0 citations ISI 02/19/14 Global Health experience 1990-1991 International Cultural Youth Exchange. Lyssach, Switzerland 1996 National Center of Reference for Rickettsiology. Marseille, France 1998-1999 Luce Scholar, Prince of Songkla University. Hat Yai, Thailand 2001 Medical Relief Officer, Kalachakra Initiation. Bodhgaya, India 2003-2004 Masters in Immunology of Infectious Diseases, LSHTM Fieldwork at the Medical Research Council Laboratory in the Gambia. 2006-2015 Collaborative research in human immunity to TB KwaZulu Natal Research Institute for TB and HIV, South Africa Language skills French (fluent), German (conversant), Thai (rusty)